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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in several tumors, which results in chromosome instability, and so may add to tumorigenesis. This protein was located to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important role in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but thorough nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified via the antibody.
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This guideline outlines more doable optimization of cellular and immunoassay parameters to be sure the absolute best results are...
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This guidebook outlines even further possible optimization of cellular and immunoassay parameters to make certain the very best final results are...
Antibodies sure to the blocking peptide now not bind to the epitope on the focus on protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, you can see which staining is specific.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterized by a remarkable periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation styles which contribute towards the temporal coordination of each mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates in the G1-S section boundary and get more info is also degraded as cells progress via S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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